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多肽合成|N端修飾

Ac

Allyl

Cholesteryl-

N-Propargylglycine-

Fmoc

Acryl

Fructose-

Ad-

CBZ

Alloc

Glucose-

BPS-

Boc

Pyr

DMAP-Ac

Morpholine-4-carbonyl-

Bz

D-Pyr

NTriAc

Trans-Cinnamoyl

Bz(5-NH2,2-NO2)

LA-

PhAc

2-Furoyl

Bz(4-F)

Mpa-

Py-Ac

Methacryl-

Bz(4-NO2)

Tos

N-Amidinoglycine

BPA-

多肽合成|N端連接脂肪酸

For

MeOSuc

5-Hexynoic acid

F-Lau-

MeOCO

Iba

heptanedioic acid

Myr-

2-Br-Ac

pentenoic acid

Oct-

F-Myr-

2-Cl-Ac

pentinoyl

F-Oct-

Pentadeconyl-

2-I-Ac

Hex-

Dec-

Pal-

OH-Ac-

5-heptenoic acid

Sebacic Acid

Cys(Pal)

3-Bromopropionyl

trans-3-hexenoyl-

F-Dec-

Pam3C-

But-

Linoleic-

Undecyl-

Ste-

Suc

10,12-Pentacosadiynoic Acid

Lau-

Octanedioic acid

多肽合成|C端修飾

-pNA

-OMe

-ONP

-OSu

-NH2

-OEt

-AMC

-NHMe

-ol

-OBzl

-AFC

-NHEt

多肽合成|D構型氨基酸

D-Ala

D-Lys

D-Glu

D-Tyr

D-Arg

D-Met

D-Gln

D-Thr

D-Asp

D-Pro

D-His

D-Trp

D-Asn

D-Phe

D-Leu

D-Val

D-Cys

D-Ser

多肽合成|熒光多肽

Biotin-

FITC-

Dansyl-

Lys(Dnp)

DeThioBiotin

-ED-FITC

-EDDansyl

D-Lys(Dnp)

-EDBiotin

-Lys(FITC)-

-Lys(Dansyl)-

Dab(Dnp)

Glu(Biotin-PEG2)

-Lys(FITC)

-Lys(Dansyl)

Dap(Dnp)

-Lys(Biotin)-

Lys(Ahx-FITC)

Dansyl-e-Lys

-EDDnp

-Lys(Biotin)

FITC-e-Lys

Dansyl-Ahx-

-Lys(LC-Biotin)-

FITC-Ahx-

5-FAM-

-Lys(LC-Biotin)

5-TAMRA-

5,6-FAM

Biotin-e-Lys

5(6)-TAMTA-

6'FAM

Lys(S-S-Biotin)

-EDTAMRA

-ED-5-FAM

Biotin-Ahx-

-Lys(TAMRA)-

-Lys(5-FAM)-

Orn(Bio)

-Lys(5-TAMRA)

Lys(5,6-FAM)

MCA-

5-TAMRA-e-Lys

-Lys(5-FAM)

-Lys(MCA)-

5-TAMRA-Acp

5-FAM-e-Lys

-Lys(MCA)

5-FAM-Ahx-

Lys(MCA)-

多肽合成|Phe及Tyr衍生物修飾

D-2-Cl-Phe

D-4-F-Phe

L-2-Me-Phe

L-3-I-Tyr

L-2-Cl-Phe

L-4-F-Phe

D-4-Me-Phe

D-3,5-DiI-Tyr

D-3-Cl-Phe

D-4-NO2-Phe

L-4-Me-Phe

L-3,5-DiI-Tyr

L-3-Cl-Phe

L-4-NO2-Phe

D-4-NH2-Phe

D-3-NO2-Tyr

D-4-Cl-Phe

D-4-I-Phe

L-4-NH2-Phe

L-3-NO2-Tyr

L-4-Cl-Phe

L-4-I-Phe

D-3-Cl-Tyr

D-3,5-DiNO2-Tyr

D-3,4-DiCl-Phe

D-3-CN-Phe

L-3-Cl-Tyr

L-3,5-DiNO2-Tyr

L-3,4-DiCl-Phe

L-3-CN-Phe

D-3,5-DiCl-Tyr

L-3-F-Tyr

D-4-Br-Phe

D-4-CN-Phe

L-3,5-DiCl-Tyr

L-4-NO2-Phg

L-4-Br-Phe

L-4-CN-Phe

D-3,5-DiBr-Tyr

D-4-NO2-Phg

D-3-F-Phe

D-2-Me-Phe

L-3,5-DiBr-Tyr

L-3,5-DiF-Phe

L-3-F-Phe

D-3-I-Tyr

多肽合成|Homo氨基酸修飾

Har

HomoCit

beta-HomoMet

beta-homoSer

D-Har

D-HomoCit

beta-HomoPro

beta-HomoThr

Hcy

HomoLeu

beta-HomoVal

beta-HomoTrp

HPh

HomoPro

beta-HomoAsp

beta-HomoTyr

D-HPh

D-HomoPro

beta-HomoAsn

Azidohomoalanine

Hse

beta-HomoIle

beta-HomoGlu

beta-HomoAla

D-Hse

beta-HomoLeu

beta-HomoGln

beta-HomoPhe

多肽合成|分支多肽修飾

Symmetric 2 Branches

Symmetric 4 Branches

Symmetric 8 Branches

Lys symmetric 2  Branches

Lys symmetric 4  Branches

Lys symmetric 8  Branches

Orn symmetric 2  Branches

Orn symmetric 4 Branches

Orn symmetric 8 Branches

Glu 2 Branches (Pure)

glycerol 3 branches ester

Thr(Peptide2)

Ser(Peptide2)

Lys(peptide2)

多肽合成|OVA,KLH,BSA蛋白偶聯

BSA-Peptide N terminus

KLH-Peptide N terminus

OVA-Peptide N terminus

BSA-peptide C terminus

KLH-Peptide C terminus

OVA-peptide C terminus

BSA-Peptide via Cys

KLH-peptide via Cys

OVA-peptide via Cys

多肽合成|Linker 連接臂修飾

Gly

Ava

AEA

GABA

Beta-Ala

Ahx

Ado

Mini-PEG

Mini-PEG2

Mini-PEG3

PEG4

多肽合成|甲基化修飾

Lys(Me)

Arg(Me)

Tyr(Me)

Tyr(Et)

Lys(Me2)

Arg(Me)2 asymmetrical

Thr(Me)

D-Tyr(Et)

Lys(Me3)

Arg(Me)2 symmetrical

Ser(Me)

多肽合成|環肽合成

一對二硫鍵

兩對二硫鍵

三對二硫鍵

三對二硫鍵隨機氧化

相同序列通過Cys分子間氧化

不同序列通過Cys分子間氧化

一對二硫鍵

Se-Cys和Se-Cys間氧化

酰胺鍵首尾成環

多肽序列通過Lys側鏈酰胺鍵成環

多肽序列通過Orn側鏈酰胺鍵成環

多肽合成|磷酸化多肽

pSer

D-pSer

Di-sites in sequence

4-sites in sequence

pTyr

D-pTyr

Tri-sites in sequence

5-sites in sequence

pThr

D-pThr

多肽合成|肽樹脂

peptide on Wang Resin

peptide on 2-CTC Resin

Peptide on Rink Amide AM Resin

peptide on Rink Amide MBHA Resin

多肽合成|測試項目

氨基酸分析

三氟醋酸含量 (HIPC)

內毒素分析

巰基測試

氮含量測試

Maldi-Tof 測試

轉成醋酸鹽

NMR核磁測試

溶解性測試

多肽合成|非天然氨基酸

Beta-Asp

3-Thi

D-Chg

Cys(pMeBzl)

D-Beta-Asp

Aib

Ac6c

Cys(pMeOBzl)

Gamma-Glu

Abu

Dab

Oic

D-Gamma-Glu

D-Abu

Dap

Tic

Cys(Cam)

Hyp

Pra

D-Tic

D-Cys(Cam)

Phg

D-Pra

Cys(Bzl)

Cys(Acm)

D-Phg

Allo-Thr

Epsilon-Lys

Cys(tBu)

Nva

D-Allo-Thr

D-Epsilon-Lys

Cys(StBu)

D-Nva

Gly(allyl)

5-ASA

Met(O)

Nle

D-Gly(allyl)

Bpa

D-Met(O)

D-Nle

Cys(Pyrene-maleimide)

Pip

Cys(SNO)

Cit

D-1-Nal

Nip

Met(O)2

D-Cit

L-1-Nal

2-Aze

D-Met(O)2

Orn

D-2-Nal

3-Aze

Lys(Ac)

D-Orn

L-2-Nal

[Psi(CH2NH)]

Ac-Lys

Pen

D-2-Pal

[CS-NH]

Lys(Dde)

D-Pen

L-2-Pal

Se-Met

Tle

Cpg

D-3-Pal

Se-Cys

Ser(octanoic acid)

Cha

L-3-Pal

DOPA

Ser(Lipoic acid)

D-Cha

D-4-Pal

isoGln

D-Ser(octanoic acid)

Chg

L-4-Pal

D-isoGln

Arg(Boc2)

Asp-OMe

D-Glu(OtBu)

Lys(Z)

Arg(NO2)

Asp-OtBu

Gln(Trt)

Ser(Bzl)

Arg(Z2)

Asn(Trt)

His(Boc)

Ser(Trt)

Arg(pbf)

Cys(MMt)

His(Trt)

Ser(tBu)

D-Arg(pbf)

Cys(Trt)

Lys(**)

Tyr(propargyl)

Asp(Oall)

D-Cys(Trt)

Lys(Boc)

Tyr(tBu)

Asp(OBn)

Glu(Oall)

Lys(DMT)

D-Tyr(tBu)

Asp(OBzl)

Glu(OBzl)

Lys(Fmoc)

Thr(tBu)

Asp(OMe)

Glu(OMe)

Lys(Mtt)

Trp(Boc)

Asp(OtBu)

Glu(OtBu)

Lys(Trt)

Trp(Farnesyl)